restriction endonuclease

1. Extraction and Restriction Endonuclease Analysis of Genomic DNA and Sequence Analysis of TK Gene from Goatpox Virus
   山羊痘病毒基因组提取、酶切及TK基因克隆测序分析
2. Studies on the Active Groups of Pst I Restriction Endonuclease
   限制性核酸内切酶PstⅠ活性基团的研究
3. Methods The restriction endonuclease and T4 DNA ligase were used to construct the vector plasmid.
   方法载体的构建采用限制性内切酶酶切、4DNA连接酶连接等方法。
4. Correlation Research between Gene Polymorphism of Vitamin D Receptor Fok ⅰ Restriction Endonuclease and Susceptivity of Gastric Cancer in Han People of Shandong Province
   山东省汉族人群维生素D受体基因FokⅠ酶切位点多态性与胃癌易感性的相关性研究
5. Choice of restriction endonuclease and establishment of silver-staining method in cDNA-AFLP System for Carthamus tinctorius
   红花cDNA-AFLP限制性内切酶的选择及银染体系的建立
6. Study of Preparation and Restriction Endonuclease of B. cepacia HMW DNA
   洋葱伯克霍尔德氏菌HMWDNA的制备及酶切研究
7. A Novel Fluorescence Assay for the Activity of Restriction Endonuclease Based on Molecular Beacon
   分子信标检测限制性内切酶活性的新型荧光分析方法
8. Methods PCR and DNA sequencing were performed to study the AR gene mutation; Mbo I restriction endonuclease was used to detect existence of the mutation in normal controls;
   并对发现突变的基因进行分析。方法应用PCR扩增、DNA序列测定等技术分析所有AR基因外显子及其邻近DNA序列片段；
9. The recombinant plasmid PET-11D-ospC was identified with restriction endonuclease analysis and sequencing.
   采用酶切分析及序列测定等方法鉴定重组质粒的正确性。
10. Result The recombinant was correctly constructed and restriction endonuclease analysis and PCR amplification.
    结果经酶切鉴定及基因测序证实重组三突变型穿梭质粒构建成功。
11. Aim To reveal the homology of the major outer-membrane protein ( MOMP) gene of ten isolates of Chlamydia psittaci, and study on the restriction endonuclease map and nucleotide sequences.
    目的进行比较性研究了10株鹦鹉热衣原体菌株，分析其主要外膜蛋白（MOMP）基因的限制性内切酶图谱和基因同源性。
12. Successful construction of AT cloning was confirmed by PCR, restriction endonuclease fragment length polymorphism analysis and sequencing.
    限制性酶切长度多肽性分析，PCR及DNA序列测定等均证实克隆构建成功。
13. Physical states of 8 HPV 11 DNA were analyzed by techniques of restriction endonuclease and Southern blot hybridization.
    用限制性内切酶和Southern转印杂交法分析了8例HPV11DNA的物理状态。
14. Results The recombinant plasmid pAd-K14-E6/ E7-polA was successfully constructed and confirmed by restriction endonuclease digestion and sequencing.
    结果通过同源重组的方法构建了腺病毒pAd-K14-E6/E7-polA载体，经酶切和测序鉴定该质粒构建成功。
15. Objective To explore the effects of the restriction endonuclease, polymerase, buffer system, BSA and DTT on strand displacement amplification ( SDA) reaction and establish SDA reaction system with commercial reagents.
    目的探索限制酶、聚合酶、缓冲体系和DTT等对链置换扩增（SDA）实验的影响，以期在商品化试剂条件下组建SDA系统。
16. In this study, a detailed restriction map was constructed using multiple groups of restriction endonuclease analysis.
    本研究利用多种组合的酶切分析，绘制了较为详细的酶切图谱。
17. A Study on Restriction Endonuclease Site Polymorphism and Haplotype in the β-globin Gene Cluster in Chinese
    中国人β-珠蛋白基因簇限制性内切酶切点多态性和单体型研究
18. The technique is useful in the analysis of restriction endonuclease digestion of genomic DNA and Southern blot.
    该法提取的血吸虫基因组DNA较纯，适合于以后进行DNA限制性核酸内切酶谱分析和Southernblot分析。
19. Methods: DNA sequencing and restriction endonuclease reaction was used.
    方法：采用DNA测序及限制性酶切反应方法。
20. The Restriction Endonuclease Analysis and Elimination of pR_ ( st98) in Salmonella typhi
    伤寒杆菌质粒pR（st98）的DNA限制性内切酶分析和药物的体外消除研究
21. The recombinant plasmid was identified by PCR amplification, digestion with restriction endonuclease and sequencing.
    重组质粒经PCR、酶切鉴定后进行测序；
22. Methods Plasmid profile and plasmid DNA restriction endonuclease analyses were carried out on 43 strains of S.flexneri 2a.
    方法对43株F2a志贺菌进行质粒图谱和质粒DNA酶切图谱分析。
23. Methods Cell culture and methylation sensitive restriction endonuclease digestion were used.
    方法采用细胞培养和限制性内切酶解分析技术观察分析细胞生长及细胞基因组DNA甲基化水平。
24. Progress on the Interaction of Restriction Endonuclease and DNA
    限制性核酸内切酶与DNA相互作用研究进展
25. The recombinant fragment of pMD-T-fbps was verified by restriction endonuclease analysis and sequencing.
    经内切酶酶切和测序鉴定后，将由pMD-T-fbps内切酶切下的片段定向克隆于表达载体pET-32a（+），得到重组质粒pfbps。
26. This was confirmed by cleavage of restriction endonuclease and DNA sequencing.
    通过质粒双酶切和DNA测序证实该重组质粒构建正确。
27. Methods Plasmid profile analysis, Plasmid DNA restriction endonuclease analysis, RAPD-PCR, Set1/ set2-PCR.
    方法对43株从患者粪便和食物中分离菌株进行质粒图谱分析、质粒DNA酶切图谱分析、随机PCR分析、set1/set2毒力基因PCR分析。
28. Restriction endonuclease and target fragment PCR detection results show that the expression vector was constructed successfully.
    经过酶切鉴定和目的片段PCR检测鉴定，证明表达载体构建成功。
29. Here we have a research on the influence of PstI restriction endonuclease on the host genomes.
    本论文是以研究PstI限制性内切酶对宿主基因组的影响为目的。
30. Traditional methods to assay the activity of restriction endonuclease are discontinuous and time consuming.
    传统的检测限制性内切酶活性的方法是不连续的，费时的。

noun

1. any of the enzymes that cut nucleic acid at specific restriction sites and produce restriction fragments

   Synonym:    restriction nucleaserestriction enzyme